4MZV

Crystal structure of extracellular part of human EpCAM

4MZV の概要

• エントリーDOI: 10.2210/pdb4mzv/pdb
• 分子名称: Epithelial cell adhesion molecule, DECYL-BETA-D-MALTOPYRANOSIDE (3 entities in total)
• 機能のキーワード: ectodomain, dimer, cell adhesion, extracellular space
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28759.55

構造登録者

Pavsic, M.,Guncar, G.,Djinovic-Carugo, K.,Lenarcic, B. (登録日: 2013-09-30, 公開日: 2014-08-27, 最終更新日: 2024-11-27)

主引用文献

Pavsic, M.,Guncar, G.,Djinovic-Carugo, K.,Lenarcic, B.
Crystal structure and its bearing towards an understanding of key biological functions of EpCAM.
Nat Commun, 5:4764-4764, 2014

PubMed Abstract: EpCAM (epithelial cell adhesion molecule), a stem and carcinoma cell marker, is a cell surface protein involved in homotypic cell-cell adhesion via intercellular oligomerization and proliferative signalling via proteolytic cleavage. Despite its use as a diagnostic marker and being a drug target, structural details of this conserved vertebrate-exclusive protein remain unknown. Here we present the crystal structure of a heart-shaped dimer of the extracellular part of human EpCAM. The structure represents a cis-dimer that would form at cell surfaces and may provide the necessary structural foundation for the proposed EpCAM intercellular trans-tetramerization mediated by a membrane-distal region. By combining biochemical, biological and structural data on EpCAM, we show how proteolytic processing at various sites could influence structural integrity, oligomeric state and associated functionality of the molecule. We also describe the epitopes of this therapeutically important protein and explain the antigenicity of its regions.

PubMed: 25163760
DOI: 10.1038/ncomms5764

実験手法

X-RAY DIFFRACTION (1.865 Å)