4MW9

Trypanosoma brucei methionyl-tRNA synthetase in complex with inhibitor 1-{3-[(3-ethynylbenzyl)amino]propyl}-3-thiophen-3-ylurea (Chem 1478)

4MW9 の概要

• エントリーDOI: 10.2210/pdb4mw9/pdb
• 関連するPDBエントリー: 4EG1 4EG3 4EG5 4MVW 4MVX 4MVY 4MW0 4MW1 4MW2 4MW4 4MW5 4MW6 4MW7 4MWB 4MWC 4MWD 4MWE
• 分子名称: Methionyl-tRNA synthetase, GLYCEROL, DIMETHYL SULFOXIDE, ... (7 entities in total)
• 機能のキーワード: aminoacyl-trna synthetase, aars, metrs, parasite, protein-inhibitor complex, rossmann fold, translation, nucleotide binding, ligase-ligase inhibitor complex, ligase/ligase inhibitor
• 由来する生物種: Trypanosoma brucei
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 124075.22

構造登録者

Koh, C.Y.,Kim, J.E.,Wetzel, A.B.,de van der Schueren, W.J.,Shibata, S.,Liu, J.,Zhang, Z.,Fan, E.,Verlinde, C.L.M.J.,Hol, W.G.J. (登録日: 2013-09-24, 公開日: 2014-04-30, 最終更新日: 2023-09-20)

主引用文献

Koh, C.Y.,Kim, J.E.,Wetzel, A.B.,de van der Schueren, W.J.,Shibata, S.,Ranade, R.M.,Liu, J.,Zhang, Z.,Gillespie, J.R.,Buckner, F.S.,Verlinde, C.L.,Fan, E.,Hol, W.G.
Structures of Trypanosoma brucei Methionyl-tRNA Synthetase with Urea-Based Inhibitors Provide Guidance for Drug Design against Sleeping Sickness.
Plos Negl Trop Dis, 8:e2775-e2775, 2014

PubMed Abstract: Methionyl-tRNA synthetase of Trypanosoma brucei (TbMetRS) is an important target in the development of new antitrypanosomal drugs. The enzyme is essential, highly flexible and displaying a large degree of changes in protein domains and binding pockets in the presence of substrate, product and inhibitors. Targeting this protein will benefit from a profound understanding of how its structure adapts to ligand binding. A series of urea-based inhibitors (UBIs) has been developed with IC50 values as low as 19 nM against the enzyme. The UBIs were shown to be orally available and permeable through the blood-brain barrier, and are therefore candidates for development of drugs for the treatment of late stage human African trypanosomiasis. Here, we expand the structural diversity of inhibitors from the previously reported collection and tested for their inhibitory effect on TbMetRS and on the growth of T. brucei cells. The binding modes and binding pockets of 14 UBIs are revealed by determination of their crystal structures in complex with TbMetRS at resolutions between 2.2 Å to 2.9 Å. The structures show binding of the UBIs through conformational selection, including occupancy of the enlarged methionine pocket and the auxiliary pocket. General principles underlying the affinity of UBIs for TbMetRS are derived from these structures, in particular the optimum way to fill the two binding pockets. The conserved auxiliary pocket might play a role in binding tRNA. In addition, a crystal structure of a ternary TbMetRS•inhibitor•AMPPCP complex indicates that the UBIs are not competing with ATP for binding, instead are interacting with ATP through hydrogen bond. This suggests a possibility that a general 'ATP-engaging' binding mode can be utilized for the design and development of inhibitors targeting tRNA synthetases of other disease-causing pathogen.

PubMed: 24743796
DOI: 10.1371/journal.pntd.0002775

実験手法

X-RAY DIFFRACTION (2.65 Å)