4MUM

Crystal structure of mitochondrial 5'(3')-deoxy ribonucleotidase alternative spliced variant

4MUM の概要

• エントリーDOI: 10.2210/pdb4mum/pdb
• 分子名称: Mitochondrial 5' nucleotidase, MAGNESIUM ION, PHOSPHATE ION, ... (9 entities in total)
• 機能のキーワード: 5'-nucleotidase, protein conformation, sequence homology, had-like, dephosphorylation, hydrolase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 25230.22

構造登録者

Pachl, P.,Rezacova, P. (登録日: 2013-09-22, 公開日: 2014-02-26, 最終更新日: 2023-09-20)

主引用文献

Pachl, P.,Fabry, M.,Veverka, V.,Brynda, J.,Rezacova, P.
Kinetic and structural characterization of an alternatively spliced variant of human mitochondrial 5'(3')-deoxyribonucleotidase.
J Enzyme Inhib Med Chem, 30:63-68, 2015

PubMed Abstract: Human mitochondrial 5'(3')-deoxyribonucleotidase (mdN) catalyzes dephosphorylation of nucleoside monophosphates, and thus helps maintain homeostasis of deoxynucleosides required for mitochondrial DNA synthesis. Mature mdN is a 23-kDa dimeric protein with highest expression levels in the heart, brain and skeletal muscle. We have identified an alternative splice variant of the mdN gene containing an 18-nucleotide insertion encoding 6 amino acids (GKWPAT) at the 3'-end of the penultimate exon 4. We recombinantly expressed this enzyme variant and characterized its biochemical and kinetic properties as well as its three-dimensional structure. Our high-resolution (1.27 Å) crystal structure revealed that the insertion forms a loop located in the vicinity of the active site pocket and affects enzyme kinetic parameters as well as protein thermal stability.

PubMed: 24506201
DOI: 10.3109/14756366.2013.879577

実験手法

X-RAY DIFFRACTION (1.271 Å)