4MT2

COMPARISON OF THE NMR SOLUTION STRUCTURE AND THE X-RAY CRYSTAL STRUCTURE OF RAT METALLOTHIONEIN-2

Replaces:  2MT2Replaces:  1MT2

Summary for 4MT2

• Entry DOI: 10.2210/pdb4mt2/pdb
• Descriptor: METALLOTHIONEIN ISOFORM II, CADMIUM ION, ZINC ION, ... (5 entities in total)
• Functional Keywords: metallothionein
• Biological source: Rattus rattus (black rat)
• Total number of polymer chains: 1
• Total formula weight: 6895.24

Authors

Robbins, A.H.,Stout, C.D. (deposition date: 1993-02-26, release date: 1993-07-15, Last modification date: 2024-11-20)

Primary citation

Braun, W.,Vasak, M.,Robbins, A.H.,Stout, C.D.,Wagner, G.,Kagi, J.H.,Wuthrich, K.
Comparison of the NMR solution structure and the x-ray crystal structure of rat metallothionein-2.
Proc.Natl.Acad.Sci.USA, 89:10124-10128, 1992

PubMed Abstract: Metallothioneins are small cysteine-rich proteins capable of binding heavy metal ions such as Zn2+ and Cd2+. They are ubiquitous tissue components in higher organisms, which tentatively have been attributed both unspecific protective functions against toxic metal ions and highly specific roles in fundamental zinc-regulated cellular processes. In this paper a detailed comparison of the NMR solution structure [Schultze, P., Wörgötter, E., Braun, W., Wagner, G., Vasák, M., Kägi, J. H. R. & Wüthrich, K. (1988) J. Mol. Biol. 203, 251-268] and a recent x-ray crystal structure [Robbins, A. H., McRee, D. E., Williamson, M., Collett, S. A., Xoung, N. H., Furey, W. F., Wang, B. C. & Stout, C. D. (1991) J. Mol. Biol. 221, 1269-1293] of rat metallothionein-2 shows that the metallothionein structures in crystals and in solution have identical molecular architectures. The structures obtained with both techniques now present a reliable basis for discussions on structure-function correlations in this class of metalloproteins.

PubMed: 1438200
DOI: 10.1073/pnas.89.21.10124

Experimental method

X-RAY DIFFRACTION (2 Å)