4MNG

Structure of the DP10.7 TCR with CD1d-sulfatide

Summary for 4MNG

• Entry DOI: 10.2210/pdb4mng/pdb
• Descriptor: Beta-2-microglobulin, Cd1d1 protein, TRA@ protein,TRA@ protein, Ti antigen CD3-associated protein gamma chain V-J-C region, ... (6 entities in total)
• Functional Keywords: immunglobulin, histocompatibility antigens, cd1, immunological, lymphocytes, gamma delta t cell, t cell recognition, self-recognition, myelin, intestinal epithelial lymphocytes, human, lipid binding, self-ligand, glycoproteins, cell-surface receptors, immune system
• Biological source: Mus musculus (Mouse); More
• Total number of polymer chains: 6
• Total formula weight: 147443.98

Authors

Luoma, A.M.,Adams, E.J. (deposition date: 2013-09-10, release date: 2013-12-18, Last modification date: 2024-10-30)

Primary citation

Luoma, A.M.,Castro, C.D.,Mayassi, T.,Bembinster, L.A.,Bai, L.,Picard, D.,Anderson, B.,Scharf, L.,Kung, J.E.,Sibener, L.V.,Savage, P.B.,Jabri, B.,Bendelac, A.,Adams, E.J.
Crystal Structure of V delta 1 T Cell Receptor in Complex with CD1d-Sulfatide Shows MHC-like Recognition of a Self-Lipid by Human gamma delta T Cells.
Immunity, 39:1032-1042, 2013

PubMed Abstract: The nature of the antigens recognized by γδ T cells and their potential recognition of major histocompatibility complex (MHC)-like molecules has remained unclear. Members of the CD1 family of lipid-presenting molecules are suggested ligands for Vδ1 TCR-expressing γδ T cells, the major γδ lymphocyte population in epithelial tissues. We crystallized a Vδ1 TCR in complex with CD1d and the self-lipid sulfatide, revealing the unusual recognition of CD1d by germline Vδ1 residues spanning all complementarity-determining region (CDR) loops, as well as sulfatide recognition separately encoded by nongermline CDR3δ residues. Binding and functional analysis showed that CD1d presenting self-lipids, including sulfatide, was widely recognized by gut Vδ1+ γδ T cells. These findings provide structural demonstration of MHC-like recognition of a self-lipid by γδ T cells and reveal the prevalence of lipid recognition by innate-like T cell populations.

PubMed: 24239091
DOI: 10.1016/j.immuni.2013.11.001

Experimental method

X-RAY DIFFRACTION (3.0058 Å)