4MGM

Crystal structure of the in vitro transcribed G. kaustophilus tRNA-Gly

4MGM の概要

• エントリーDOI: 10.2210/pdb4mgm/pdb
• 関連するPDBエントリー: 4MGN
• 分子名称: tRNA glycine, MAGNESIUM ION (2 entities in total)
• 機能のキーワード: rna-rna complex, base stacking, t-loop, rna binding, rna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 48684.94

構造登録者

Grigg, J.C.,Ke, A. (登録日: 2013-08-28, 公開日: 2013-10-23, 最終更新日: 2023-09-20)

主引用文献

Grigg, J.C.,Ke, A.
Structural Determinants for Geometry and Information Decoding of tRNA by T Box Leader RNA.
Structure, 21:2025-2032, 2013

PubMed Abstract: T box riboswitches are cis-acting RNA elements that bind to tRNA and sense its aminoacylation state to influence gene expression. Here, we present the 3.2 Å resolution X-ray crystal structures of the T box Stem I-tRNA complex and tRNA, in isolation. T box Stem I forms an arched conformation with extensive intermolecular contacts to two key points of tRNA, the anticodon and D/T-loops. Free and complexed tRNA exist in significantly different conformations, with the contacts stabilizing flexible D/T-loops and a rearrangement of the D-loop. Using a designed T box RNA/tRNA system, we demonstrate that the T box riboswitch monitors the length and orientation of two essential contacts. Length or orientation mismatches engineered into the T box riboswitch and tRNA disrupt the complex, whereas simultaneous insertion of full helical turns realigns the interfaces and restores interaction between artificially elongated T box riboswitch and tRNA molecules.

PubMed: 24095061
DOI: 10.1016/j.str.2013.09.001

実験手法

X-RAY DIFFRACTION (3.2 Å)