The structure of E292S glycosynthase variant of exo-1,3-beta-glucanase from Candida albicans complexed with p-nitrophenyl-gentiobioside (product) at 1.6A resolution

Summary for 4M82

Related1CZ1 4M80 4M81
DescriptorEXO-1,3-BETA-GLUCANASE, 4-nitrophenyl 6-O-beta-D-glucopyranosyl-beta-D-glucopyranoside, BETA-D-GLUCOSE, ... (5 entities in total)
Functional Keywordstim barrel, glycoside hydrolase family 5, glycoside hydrolase, cell wall hydrolase, glycosynthase, hydrolase, protein-carbohydrate interaction
Biological sourceCandida albicans (yeast)
Total number of polymer chains1
Total molecular weight46460.99
Nakatani, Y.,Cutfield, S.M.,Larsen, D.S.,Cutfield, J.F. (deposition date: 2013-08-12, release date: 2014-06-25)
Primary citation
Nakatani, Y.,Larsen, D.S.,Cutfield, S.M.,Cutfield, J.F.
Major Change in Regiospecificity for the Exo-1,3-beta-glucanase from Candida albicans following Its Conversion to a Glycosynthase.
Biochemistry, 53:3318-3326, 2014
PubMed: 24804868 (PDB entries with the same primary citation)
DOI: 10.1021/bi500239m
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.18420.3%0.3%1.0%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation report