4M63

Crystal Structure of a Filament-Like Actin Trimer Bound to the Bacterial Effector VopL

4M63 の概要

• エントリーDOI: 10.2210/pdb4m63/pdb
• 分子名称: T3SS2 effector VopL nucleation of actin polymerization, Actin-5C, ADENOSINE-5'-TRIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: actin nucleator, actin nucleation, hydrolase, wasp homology 2 domain, vopl c-terminal domain, cytoskeleton, atp-binding protein, actin-binding protein
• 由来する生物種: Vibrio parahaemolyticus; 詳細
• 細胞内の位置: Cytoplasm, cytoskeleton: P10987
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 180700.27

構造登録者

Tomchick, D.R.,Zahm, J.A.,Rosen, M.K. (登録日: 2013-08-08, 公開日: 2013-10-23, 最終更新日: 2023-09-20)

主引用文献

Zahm, J.A.,Padrick, S.B.,Chen, Z.,Pak, C.W.,Yunus, A.A.,Henry, L.,Tomchick, D.R.,Chen, Z.,Rosen, M.K.
The Bacterial Effector VopL Organizes Actin into Filament-like Structures.
Cell(Cambridge,Mass.), 155:423-434, 2013

PubMed Abstract: VopL is an effector protein from Vibrio parahaemolyticus that nucleates actin filaments. VopL consists of a VopL C-terminal domain (VCD) and an array of three WASP homology 2 (WH2) motifs. Here, we report the crystal structure of the VCD dimer bound to actin. The VCD organizes three actin monomers in a spatial arrangement close to that found in the canonical actin filament. In this arrangement, WH2 motifs can be modeled into the binding site of each actin without steric clashes. The data suggest a mechanism of nucleation wherein VopL creates filament-like structures, organized by the VCD with monomers delivered by the WH2 array, that can template addition of new subunits. Similarities with Arp2/3 complex and formin proteins suggest that organization of monomers into filament-like structures is a general and central feature of actin nucleation.

PubMed: 24120140
DOI: 10.1016/j.cell.2013.09.019

実験手法

X-RAY DIFFRACTION (2.748 Å)