4M30

Crystal structure of RNASE III complexed with double-stranded RNA AND AMP (TYPE II CLEAVAGE)

4M30 の概要

• エントリーDOI: 10.2210/pdb4m30/pdb
• 関連するPDBエントリー: 2NUG 4M2Z
• 分子名称: Ribonuclease 3, RNA12, MAGNESIUM ION, ... (6 entities in total)
• 機能のキーワード: rnase iii, hydrolase, dsrna, rna binding, rna processing, hydrolase-rna complex, hydrolase/rna
• 由来する生物種: Aquifex aeolicus; 詳細
• 細胞内の位置: Cytoplasm (By similarity): O67082
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 70628.44

構造登録者

Gan, J.,Liang, Y.-H.,Shaw, G.X.,Tropea, J.E.,Waugh, D.S.,Ji, X. (登録日: 2013-08-05, 公開日: 2013-12-11, 最終更新日: 2024-05-29)

主引用文献

Court, D.L.,Gan, J.,Liang, Y.H.,Shaw, G.X.,Tropea, J.E.,Costantino, N.,Waugh, D.S.,Ji, X.
RNase III: Genetics and Function; Structure and Mechanism.
Annu. Rev. Genet., 47:405-431, 2013

PubMed Abstract: RNase III is a global regulator of gene expression in Escherichia coli that is instrumental in the maturation of ribosomal and other structural RNAs. We examine here how RNase III itself is regulated in response to growth and other environmental changes encountered by the cell and how, by binding or processing double-stranded RNA (dsRNA) intermediates, RNase III controls the expression of genes. Recent insight into the mechanism of dsRNA binding and processing, gained from structural studies of RNase III, is reviewed. Structural studies also reveal new cleavage sites in the enzyme that can generate longer 3' overhangs.

PubMed: 24274754
DOI: 10.1146/annurev-genet-110711-155618

実験手法

X-RAY DIFFRACTION (2.501 Å)