4LVR

Crystal structure of IFT81 N-terminal domain

Summary for 4LVR

• Entry DOI: 10.2210/pdb4lvr/pdb
• Related: 4LVP
• Descriptor: Intraflagellar transport protein 81 (1 entity in total)
• Functional Keywords: ch domain, tubulin/microtubules, transport protein
• Biological source: Chlamydomonas reinhardtii
• Total number of polymer chains: 1
• Total formula weight: 14175.35

Authors

Bhogaraju, S. (deposition date: 2013-07-26, release date: 2013-09-11, Last modification date: 2024-02-28)

Primary citation

Bhogaraju, S.,Cajanek, L.,Fort, C.,Blisnick, T.,Weber, K.,Taschner, M.,Mizuno, N.,Lamla, S.,Bastin, P.,Nigg, E.A.,Lorentzen, E.
Molecular Basis of Tubulin Transport Within the Cilium by IFT74 and IFT81.
Science, 341:1009-1012, 2013

PubMed Abstract: Intraflagellar transport (IFT) of ciliary precursors such as tubulin from the cytoplasm to the ciliary tip is involved in the construction of the cilium, a hairlike organelle found on most eukaryotic cells. However, the molecular mechanisms of IFT are poorly understood. Here, we found that the two core IFT proteins IFT74 and IFT81 form a tubulin-binding module and mapped the interaction to a calponin homology domain of IFT81 and a highly basic domain in IFT74. Knockdown of IFT81 and rescue experiments with point mutants showed that tubulin binding by IFT81 was required for ciliogenesis in human cells.

PubMed: 23990561
DOI: 10.1126/science.1240985

Experimental method

X-RAY DIFFRACTION (2.6 Å)