4LUQ

Crystal structure of virulence effector Tse3 in complex with neutralizer Tsi3

Summary for 4LUQ

• Entry DOI: 10.2210/pdb4luq/pdb
• Descriptor: Uncharacterized protein, CALCIUM ION, ... (4 entities in total)
• Functional Keywords: goose type lysozyme, catalytic domain, arm, heat like motif, muramidase, tsi3, protein binding-toxin inhibitor complex, protein binding/toxin inhibitor
• Biological source: Pseudomonas aeruginosa; More
• Total number of polymer chains: 4
• Total formula weight: 125366.48

Authors

Wang, T.,Li, L.,Zhang, W. (deposition date: 2013-07-25, release date: 2013-09-18, Last modification date: 2024-03-20)

Primary citation

Li, L.,Zhang, W.,Liu, Q.,Gao, Y.,Gao, Y.,Wang, Y.,Wang, D.Z.,Li, Z.,Wang, T.
Structural Insights on the Bacteriolytic and Self-protection Mechanism of Muramidase Effector Tse3 in Pseudomonas aeruginosa
J.Biol.Chem., 288:30607-30613, 2013

PubMed Abstract: The warfare among microbial species as well as between pathogens and hosts is fierce, complicated, and continuous. In Pseudomonas aeruginosa, the muramidase effector Tse3 (Type VI secretion exported 3) can be injected into the periplasm of neighboring bacterial competitors by a Type VI secretion apparatus, eventually leading to cell lysis and death. However, P. aeruginosa protects itself from lysis by expressing immune protein Tsi3 (Type six secretion immunity 3). Here, we report the crystal structure of the Tse3-Tsi3 complex at 1.8 Å resolution, revealing that Tse3 possesses one open accessible, goose-type lysozyme-like domain with peptidoglycan hydrolysis activity. Calcium ions bind specifically in the Tse3 active site and are identified to be crucial for its bacteriolytic activity. In combination with biochemical studies, the structural basis of self-protection mechanism of Tsi3 is also elucidated, thus providing an understanding and new insights into the effectors of Type VI secretion system.

PubMed: 24025333
DOI: 10.1074/jbc.C113.506097

Experimental method

X-RAY DIFFRACTION (1.77 Å)