4LE9

Crystal structure of a chimeric c-Src-SH3 domain

4LE9 の概要

• エントリーDOI: 10.2210/pdb4le9/pdb
• 関連するPDBエントリー: 3FJ5 4JZ3 4JZ4
• 分子名称: Proto-oncogene tyrosine-protein kinase Src, TRIETHYLENE GLYCOL (3 entities in total)
• 機能のキーワード: beta shandwich, sh3, kinase, proly proline rich motifs, phosphorylation, transferase
• 由来する生物種: Gallus gallus (bantam,chickens)
• 細胞内の位置: Cell membrane (By similarity): P00523
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8827.73

構造登録者

Camara-Artigas, A.,Martinez-Rodriguez, S.,Ortiz-Salmeron, E.,Martin-Garcia, J.M. (登録日: 2013-06-25, 公開日: 2014-05-07, 最終更新日: 2023-09-20)

主引用文献

Camara-Artigas, A.,Martinez-Rodriguez, S.,Ortiz-Salmeron, E.,Martin-Garcia, J.M.
3D domain swapping in a chimeric c-Src SH3 domain takes place through two hinge loops.
J.Struct.Biol., 186:195-203, 2014

PubMed Abstract: In the Src Homology 3 domain (SH3) the RT and n-Src loops form a pocket that accounts for the specificity and affinity in binding of proline rich motifs (PRMs), while the distal and diverging turns play a key role in the folding of the protein. We have solved the structure of a chimeric mutant c-Src-SH3 domain where specific residues at the RT- and n-Src-loops have been replaced by those present in the corresponding Abl-SH3 domain. Crystals of the chimeric protein show a single molecule in the asymmetric unit, which appears in an unfolded-like structure that upon generation of the symmetry related molecules reveals the presence of a domain swapped dimer where both, RT- and n-Src loops, act as hinge loops. In contrast, the fold of the diverging type II β-turn and the distal loop are well conserved. Our results are the first evidence for the presence of a structured diverging type II β-turn in an unfolded-like intermediate of the c-Src-SH3 domain, which can be stabilized by interactions from the β-strands of the same polypeptide chain or from a neighboring one. Futhermore, this crystallographic structure opens a unique opportunity to study the effect of the amino acid sequence of the hinge loops on the 3D domain swapping process of c-Src-SH3.

PubMed: 24556574
DOI: 10.1016/j.jsb.2014.02.007

実験手法

X-RAY DIFFRACTION (1.344 Å)