4L6A

Structure of human mitochondrial 5'(3')-deoxyribonucleotidase

4L6A の概要

• エントリーDOI: 10.2210/pdb4l6a/pdb
• 関連するPDBエントリー: 4L6C
• 分子名称: 5'(3')-deoxyribonucleotidase, mitochondrial, MAGNESIUM ION, PHOSPHATE ION, ... (7 entities in total)
• 機能のキーワード: protein conformation, sequence homology, had-like, hydrolase, dephosphorylation, phosphorylation
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Mitochondrion : Q9NPB1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24683.23

構造登録者

Pachl, P.,Brynda, J.,Rezacova, P. (登録日: 2013-06-12, 公開日: 2014-02-12, 最終更新日: 2024-02-28)

主引用文献

Pachl, P.,Fabry, M.,Rosenberg, I.,Simak, O.,Rezacova, P.,Brynda, J.
Structures of human cytosolic and mitochondrial nucleotidases: implications for structure-based design of selective inhibitors.
Acta Crystallogr. D Biol. Crystallogr., 70:461-470, 2014

PubMed Abstract: The human 5'(3')-deoxyribonucleotidases catalyze the dephosphorylation of deoxyribonucleoside monophosphates to the corresponding deoxyribonucleosides and thus help to maintain the balance between pools of nucleosides and nucleotides. Here, the structures of human cytosolic deoxyribonucleotidase (cdN) at atomic resolution (1.08 Å) and mitochondrial deoxyribonucleotidase (mdN) at near-atomic resolution (1.4 Å) are reported. The attainment of an atomic resolution structure allowed interatomic distances to be used to assess the probable protonation state of the phosphate anion and the side chains in the enzyme active site. A detailed comparison of the cdN and mdN active sites allowed the design of a cdN-specific inhibitor.

PubMed: 24531480
DOI: 10.1107/S1399004713030502

実験手法

X-RAY DIFFRACTION (1.4 Å)