4L25

Crystal structure of DNA duplex containing consecutive T-T mispairs

4L25 の概要

• エントリーDOI: 10.2210/pdb4l25/pdb
• 関連するPDBエントリー: 4L24 4L26
• 分子名称: DNA (5'-D(*CP*GP*CP*GP*AP*TP*TP*TP*CP*GP*CP*G)-3') (2 entities in total)
• 機能のキーワード: t-t mispair, a-t-t triplet, non-helical dna duplex, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 7308.76

構造登録者

Kondo, J.,Yamada, T.,Hirose, C.,Tanaka, Y.,Ono, A. (登録日: 2013-06-04, 公開日: 2014-03-05, 最終更新日: 2023-11-08)

主引用文献

Kondo, J.,Yamada, T.,Hirose, C.,Okamoto, I.,Tanaka, Y.,Ono, A.
Crystal Structure of Metallo DNA Duplex Containing Consecutive Watson-Crick-like T-Hg(II) -T Base Pairs
Angew.Chem.Int.Ed.Engl., 53:2385-2388, 2014

PubMed Abstract: The metallo DNA duplex containing mercury-mediated T-T base pairs is an attractive biomacromolecular nanomaterial which can be applied to nanodevices such as ion sensors. Reported herein is the first crystal structure of a B-form DNA duplex containing two consecutive T-Hg(II)-T base pairs. The Hg(II) ion occupies the center between two T residues. The N3-Hg(II) bond distance is 2.0 Å. The relatively short Hg(II)-Hg(II) distance (3.3 Å) observed in consecutive T-Hg(II)-T base pairs suggests that the metallophilic attraction could exist between them and may stabilize the B-form double helix. To support this, the DNA duplex is largely distorted and adopts an unusual nonhelical conformation in the absence of Hg(II). The structure of the metallo DNA duplex itself and the Hg(II)-induced structural switching from the nonhelical form to the B-form provide the basis for structure-based design of metal-conjugated nucleic acid nanomaterials.

PubMed: 24478025
DOI: 10.1002/anie.201309066

実験手法

X-RAY DIFFRACTION (1.1 Å)