4KZG

Crystal structure of zebrafish MO25

Summary for 4KZG

• Entry DOI: 10.2210/pdb4kzg/pdb
• Descriptor: Zgc:86716 (2 entities in total)
• Functional Keywords: scaffold protein, signaling protein, kinase activator, ste20 kinases
• Biological source: Danio rerio (Zebrafish)
• Total number of polymer chains: 8
• Total formula weight: 304830.75

Authors

Zhang, Z.Z.,Shi, Z.B.,Zhang, M. (deposition date: 2013-05-30, release date: 2013-09-11, Last modification date: 2023-11-08)

Primary citation

Zhang, Z.Z.,Wang, Y.C.,Shi, Z.B.,Zhang, M.
Structure of zebrafish MO25
Acta Crystallogr.,Sect.F, 69:989-993, 2013

PubMed Abstract: MO25, a conserved scaffold protein, activates the tumour suppressor LKB1 with the pseudokinase STRAD. MO25 also promotes the activities of the STE20-family kinases MST3, MST4, STK25, SPAK and OSR1. Zebrafish MO25 was purified and crystallized, and a crystal of zebrafish MO25 diffracted to 2.9 Å resolution and belonged to space group P3221, with unit-cell parameters a = b = 156.665, c = 221.251 Å. The structure of zebrafish MO25 was determined by molecular replacement. It is constituted of seven helical repeats. Structural comparison indicates that the overall structures of zebrafish and human MO25 are very similar, suggesting that MO25 has conserved functions in zebrafish. This work provides a structural basis for further functional and evolutionary studies of MO25.

PubMed: 23989145
DOI: 10.1107/S1744309113021520

Experimental method

X-RAY DIFFRACTION (2.9 Å)