4KW6

Crystal structure of Peroxiredoxin-1 (C-terminal truncation mutant) from the human hookworm Ancylostoma ceylanicum bound to conoidin A

Summary for 4KW6

• Entry DOI: 10.2210/pdb4kw6/pdb
• Related: 4FH8
• Descriptor: Peroxiredoxin-1, 2,3-bis(bromomethyl)quinoxaline 1,4-dioxide (3 entities in total)
• Functional Keywords: 2-cys peroxiredoxin, oxidoreductase-oxidoreductase inhibitor complex, oxidoreductase/oxidoreductase inhibitor
• Biological source: Ancylostoma ceylanicum
• Total number of polymer chains: 5
• Total formula weight: 101851.93

Authors

Nguyen, J.B.,Modis, Y. (deposition date: 2013-05-23, release date: 2013-07-31, Last modification date: 2024-11-06)

Primary citation

Nguyen, J.B.,Pool, C.D.,Wong, C.Y.,Treger, R.S.,Williams, D.L.,Cappello, M.,Lea, W.A.,Simeonov, A.,Vermeire, J.J.,Modis, Y.
Peroxiredoxin-1 from the Human Hookworm Ancylostoma ceylanicum Forms a Stable Oxidized Decamer and Is Covalently Inhibited by Conoidin A.
Chem.Biol., 20:991-1001, 2013

PubMed Abstract: Hookworms are parasitic nematodes that have a devastating impact on global health, particularly in developing countries. We report a biochemical and structural analysis of a peroxiredoxin from the hookworm Ancylostoma ceylanicum, AcePrx-1. Peroxiredoxins provide antioxidant protection and act as signaling molecules and chaperones. AcePrx-1 is expressed in adult hookworms and can be inactivated by 2,3-bis(bromomethyl)quinoxaline-1,4-dioxide (conoidin A). Conoidin A inactivates AcePrx-1 by alkylating or crosslinking the catalytic cysteines, while maintaining the enzyme in the "locally unfolded" conformation. Irreversible oxidation of the resolving cysteine may contribute additional inhibitory activity. A crystal structure of oxidized AcePrx-1 reveals a disulfide-linked decamer. A helix macrodipole near the active site increases the reactivity of the catalytic cysteines to conoidin A. This work demonstrates the promise of conoidin compounds as probes to evaluate peroxiredoxins as drug targets in human parasites.

PubMed: 23891152
DOI: 10.1016/j.chembiol.2013.06.011

Experimental method

X-RAY DIFFRACTION (3 Å)