4KSC

Structures of P-glycoprotein reveal its conformational flexibility and an epitope on the nucleotide-binding domain

Summary for 4KSC

• Entry DOI: 10.2210/pdb4ksc/pdb
• Related: 4KSB 4KSD 4LSG
• Descriptor: Multidrug resistance protein 1A (1 entity in total)
• Functional Keywords: transporter, atp binding, membrane, membrane protein, transport protein
• Biological source: Mus musculus (mouse)
• Total number of polymer chains: 1
• Total formula weight: 141877.88

Authors

Ward, A.,Szewczyk, P.,Grimard, V.,Lee, C.-W.,Martinez, L.,Doshi, R.,Caya, A.,Villaluz, M.,Pardon, E.,Cregger, C.,Swartz, D.J.,Falson, P.,Urbatsch, I.,Govaerts, C.,Steyaert, J.,Chang, G. (deposition date: 2013-05-17, release date: 2013-07-31, Last modification date: 2024-02-28)

Primary citation

Ward, A.B.,Szewczyk, P.,Grimard, V.,Lee, C.W.,Martinez, L.,Doshi, R.,Caya, A.,Villaluz, M.,Pardon, E.,Cregger, C.,Swartz, D.J.,Falson, P.G.,Urbatsch, I.L.,Govaerts, C.,Steyaert, J.,Chang, G.
Structures of P-glycoprotein reveal its conformational flexibility and an epitope on the nucleotide-binding domain.
Proc.Natl.Acad.Sci.USA, 110:13386-13391, 2013

PubMed Abstract: P-glycoprotein (P-gp) is one of the best-known mediators of drug efflux-based multidrug resistance in many cancers. This validated therapeutic target is a prototypic, plasma membrane resident ATP-Binding Cassette transporter that pumps xenobiotic compounds out of cells. The large, polyspecific drug-binding pocket of P-gp recognizes a variety of structurally unrelated compounds. The transport of these drugs across the membrane is coincident with changes in the size and shape of this pocket during the course of the transport cycle. Here, we present the crystal structures of three inward-facing conformations of mouse P-gp derived from two different crystal forms. One structure has a nanobody bound to the C-terminal side of the first nucleotide-binding domain. This nanobody strongly inhibits the ATP hydrolysis activity of mouse P-gp by hindering the formation of a dimeric complex between the ATP-binding domains, which is essential for nucleotide hydrolysis. Together, these inward-facing conformational snapshots of P-gp demonstrate a range of flexibility exhibited by this transporter, which is likely an essential feature for the binding and transport of large, diverse substrates. The nanobody-bound structure also reveals a unique epitope on P-gp.

PubMed: 23901103
DOI: 10.1073/pnas.1309275110

Experimental method

X-RAY DIFFRACTION (4 Å)