4KPI
Rotational order-disorder structure of reversibly photoswitchable red fluorescent protein rsTagRFP
Summary for 4KPI
| Entry DOI | 10.2210/pdb4kpi/pdb |
| Related | 3U8A 3U8C |
| Descriptor | Reversibly photoswitchable red fluorescent protein rsTagRFP (2 entities in total) |
| Functional Keywords | beta-barrel, order-disorder structure, red fluorescent protein, reversibly photoswitchable fluorescent protein, fluorescent protein |
| Biological source | synthetic construct |
| Total number of polymer chains | 1 |
| Total formula weight | 27639.46 |
| Authors | Pletnev, S. (deposition date: 2013-05-13, release date: 2014-02-26, Last modification date: 2023-12-06) |
| Primary citation | Pletnev, S.,Subach, F.V.,Verkhusha, V.V.,Dauter, Z. The rotational order-disorder structure of the reversibly photoswitchable red fluorescent protein rsTagRFP. Acta Crystallogr.,Sect.D, 70:31-39, 2014 Cited by PubMed Abstract: The rotational order-disorder (OD) structure of the reversibly photoswitchable fluorescent protein rsTagRFP is discussed in detail. The structure is composed of tetramers of 222 symmetry incorporated into the lattice in two different orientations rotated 90° with respect to each other around the crystal c axis and with tetramer axes coinciding with the crystallographic twofold axes. The random distribution of alternatively oriented tetramers in the crystal creates the rotational OD structure with statistically averaged I422 symmetry. Despite order-disorder pathology, the structure of rsTagRFP has electron-density maps of good quality for both non-overlapping and overlapping parts of the model. The crystal contacts, crystal internal architecture and a possible mechanism of rotational OD crystal formation are discussed. PubMed: 24419376DOI: 10.1107/S1399004713024644 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.58 Å) |
Structure validation
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