4KP3

Crystal Structure of MyoVa-GTD in Complex with Two Cargos

Summary for 4KP3

• Entry DOI: 10.2210/pdb4kp3/pdb
• Related: 3WB8
• Descriptor: Unconventional myosin-Va, RILP-like protein 2, Melanophilin, ... (4 entities in total)
• Functional Keywords: helix bundle, motor protein-protein transport complex, motor protein/protein transport
• Biological source: Mus musculus (mouse); More
• Cellular location: Cytoplasm, cytosol : Q99LE1; Melanosome: Q91V27
• Total number of polymer chains: 6
• Total formula weight: 125193.13

Authors

Wei, Z.,Liu, X.,Yu, C.,Zhang, M. (deposition date: 2013-05-13, release date: 2013-07-10, Last modification date: 2023-11-08)

Primary citation

Wei, Z.,Liu, X.,Yu, C.,Zhang, M.
Structural basis of cargo recognitions for class V myosins
Proc.Natl.Acad.Sci.USA, 110:11314-11319, 2013

PubMed Abstract: Class V myosins (MyoV), the most studied unconventional myosins, recognize numerous cargos mainly via the motor's globular tail domain (GTD). Little is known regarding how MyoV-GTD recognizes such a diverse array of cargos specifically. Here, we solved the crystal structures of MyoVa-GTD in its apo-form and in complex with two distinct cargos, melanophilin and Rab interacting lysosomal protein-like 2. The apo-MyoVa-GTD structure indicates that most mutations found in patients with Griscelli syndrome, microvillus inclusion disease, or cancers or in "dilute" rodents likely impair the folding of GTD. The MyoVa-GTD/cargo complex structure reveals two distinct cargo-binding surfaces, one primarily via charge-charge interaction and the other mainly via hydrophobic interactions. Structural and biochemical analysis reveal the specific cargo-binding specificities of various isoforms of mammalian MyoV as well as very different cargo recognition mechanisms of MyoV between yeast and higher eukaryotes. The MyoVa-GTD structures resolved here provide a framework for future functional studies of vertebrate class V myosins.

PubMed: 23798443
DOI: 10.1073/pnas.1306768110

Experimental method

X-RAY DIFFRACTION (2.405 Å)