4KHP

Structure of the Thermus thermophilus 30S ribosomal subunit in complex with de-6-MSA-pactamycin

4KHP の概要

• エントリーDOI: 10.2210/pdb4khp/pdb
• 関連するPDBエントリー: 1HNX
• 分子名称: 16S Ribosomal RNA, 30S Ribosomal protein S10, 30S Ribosomal protein S11, ... (25 entities in total)
• 機能のキーワード: pactamycin, 30s, mrna, e site, trna, ribosome, translation, antibiotic, ribosome-antibiotic complex, de-6-msa-pactamycin and pactamycin analog, ribosome/antibiotic
• 由来する生物種: Thermus thermophilus; 詳細
• タンパク質・核酸の鎖数: 22
• 化学式量合計: 770160.41

構造登録者

Tourigny, D.S.,Fernandez, I.S.,Kelley, A.C.,Ramakrishnan, V. (登録日: 2013-05-01, 公開日: 2013-06-05, 最終更新日: 2024-11-06)

主引用文献

Tourigny, D.S.,Fernandez, I.S.,Kelley, A.C.,Vakiti, R.R.,Chattopadhyay, A.K.,Dorich, S.,Hanessian, S.,Ramakrishnan, V.
Crystal Structure of a Bioactive Pactamycin Analog Bound to the 30S Ribosomal Subunit.
J.Mol.Biol., 425:3907-3910, 2013

PubMed Abstract: Biosynthetically and chemically derived analogs of the antibiotic pactamycin and de-6-methylsalicylyl (MSA)-pactamycin have attracted recent interest as potential antiprotozoal and antitumor drugs. Here, we report a 3.1-Å crystal structure of de-6-MSA-pactamycin bound to its target site on the Thermus thermophilus 30S ribosomal subunit. Although de-6-MSA-pactamycin lacks the MSA moiety, it shares the same binding site as pactamycin and induces a displacement of nucleic acid template bound at the E-site of the 30S. The structure highlights unique interactions between this pactamycin analog and the ribosome, which paves the way for therapeutic development of related compounds.

PubMed: 23702293
DOI: 10.1016/j.jmb.2013.05.004

実験手法

X-RAY DIFFRACTION (3.1 Å)