4KGM

Bacterial tRNA(HIS) Guanylyltransferase (Thg1)-Like Protein in complex with ATP

4KGM の概要

• エントリーDOI: 10.2210/pdb4kgm/pdb
• 関連するPDBエントリー: 4KGK
• 分子名称: Thg1-like uncharacterized protein, ADENOSINE-5'-TRIPHOSPHATE, PHOSPHATE ION, ... (6 entities in total)
• 機能のキーワード: polymerase palm-like catalytic domain, transferase, gtp and trna binding
• 由来する生物種: Bacillus thuringiensis
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 117021.17

構造登録者

Hyde, S.J.,Rao, B.S.,Eckenroth, B.E.,Jackman, J.E.,Doublie, S. (登録日: 2013-04-29, 公開日: 2013-08-14, 最終更新日: 2024-02-28)

主引用文献

Hyde, S.J.,Rao, B.S.,Eckenroth, B.E.,Jackman, J.E.,Doublie, S.
Structural Studies of a Bacterial tRNA(HIS) Guanylyltransferase (Thg1)-Like Protein, with Nucleotide in the Activation and Nucleotidyl Transfer Sites.
Plos One, 8:e67465-e67465, 2013

PubMed Abstract: All nucleotide polymerases and transferases catalyze nucleotide addition in a 5' to 3' direction. In contrast, tRNA(His) guanylyltransferase (Thg1) enzymes catalyze the unusual reverse addition (3' to 5') of nucleotides to polynucleotide substrates. In eukaryotes, Thg1 enzymes use the 3'-5' addition activity to add G-1 to the 5'-end of tRNA(His), a modification required for efficient aminoacylation of the tRNA by the histidyl-tRNA synthetase. Thg1-like proteins (TLPs) are found in Archaea, Bacteria, and mitochondria and are biochemically distinct from their eukaryotic Thg1 counterparts TLPs catalyze 5'-end repair of truncated tRNAs and act on a broad range of tRNA substrates instead of exhibiting strict specificity for tRNA(His). Taken together, these data suggest that TLPs function in distinct biological pathways from the tRNA(His) maturation pathway, perhaps in tRNA quality control. Here we present the first crystal structure of a TLP, from the gram-positive soil bacterium Bacillus thuringiensis (BtTLP). The enzyme is a tetramer like human THG1, with which it shares substantial structural similarity. Catalysis of the 3'-5' reaction with 5'-monophosphorylated tRNA necessitates first an activation step, generating a 5'-adenylylated intermediate prior to a second nucleotidyl transfer step, in which a nucleotide is transferred to the tRNA 5'-end. Consistent with earlier characterization of human THG1, we observed distinct binding sites for the nucleotides involved in these two steps of activation and nucleotidyl transfer. A BtTLP complex with GTP reveals new interactions with the GTP nucleotide in the activation site that were not evident from the previously solved structure. Moreover, the BtTLP-ATP structure allows direct observation of ATP in the activation site for the first time. The BtTLP structural data, combined with kinetic analysis of selected variants, provide new insight into the role of key residues in the activation step.

PubMed: 23844012
DOI: 10.1371/journal.pone.0067465

実験手法

X-RAY DIFFRACTION (2.361 Å)