4KGM
Bacterial tRNA(HIS) Guanylyltransferase (Thg1)-Like Protein in complex with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-04-26 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.03 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 99.156, 218.343, 126.056 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.700 - 2.361 |
| R-factor | 0.2021 |
| Rwork | 0.199 |
| R-free | 0.23210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.720 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.450 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 56361 | |
| Completeness [%] | 99.7 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 13% (w/v) polyethylene glycol 4000, 50 mM MgSO4 and 100 mM Tris(2-carboxyethyl) phosphine, pH 7 and ~1 mM ATP, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
