4KFC

Crystal structure of a hyperactive mutant of response regulator KdpE complexed to its promoter DNA

4KFC の概要

• エントリーDOI: 10.2210/pdb4kfc/pdb
• 分子名称: KDP operon transcriptional regulatory protein KdpE, Promoter DNA, ... (4 entities in total)
• 機能のキーワード: receiver domain, dna-binding domain, transcription regulator-dna complex, transcription regulator/dna
• 由来する生物種: Escherichia coli; 詳細
• 細胞内の位置: Cytoplasm: P21866
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 69318.34

構造登録者

Kumar, S.,Narayanan, A.,Yernool, D.A. (登録日: 2013-04-26, 公開日: 2014-02-19, 最終更新日: 2023-09-20)

主引用文献

Narayanan, A.,Kumar, S.,Evrard, A.N.,Paul, L.N.,Yernool, D.A.
An asymmetric heterodomain interface stabilizes a response regulator-DNA complex.
Nat Commun, 5:3282-3282, 2014

PubMed Abstract: Two-component signal transduction systems consist of pairs of histidine kinases and response regulators, which mediate adaptive responses to environmental cues. Most activated response regulators regulate transcription by binding tightly to promoter DNA via a phosphorylation-triggered inactive-to-active transition. The molecular basis for formation of stable response regulator-DNA complexes that precede the assembly of RNA polymerases is unclear. Here, we present structures of DNA complexed with the response regulator KdpE, a member of the OmpR/PhoB family. The distinctively asymmetric complex in an active-like conformation reveals a unique intramolecular interface between the receiver domain (RD) and the DNA-binding domain (DBD) of only one of the two response regulators in the complex. Structure-function studies show that this RD-DBD interface is necessary to form stable complexes that support gene expression. The conservation of sequence and structure suggests that these findings extend to a large group of response regulators that act as transcription factors.

PubMed: 24526190
DOI: 10.1038/ncomms4282

実験手法

X-RAY DIFFRACTION (2.53 Å)