4K7Q

Crystal Structure of AcrB Complexed with Linezolid at 3.5 Resolution

Summary for 4K7Q

Entry DOI	10.2210/pdb4k7q/pdb
Descriptor	Acriflavine resistance protein B, N-{[(5S)-3-(3-fluoro-4-morpholin-4-ylphenyl)-2-oxo-1,3-oxazolidin-5-yl]methyl}acetamide (2 entities in total)
Functional Keywords	inner membrane resistance-nodulation-cell division efflux pump, membrane protein-antibiotic complex, membrane protein/antibiotic
Biological source	Escherichia coli
Cellular location	Cell inner membrane ; Multi- pass membrane protein : P31224
Total number of polymer chains	1
Total formula weight	114555.09
Authors	Hung, L.W.,Kim, H.B.,Murakami, S.,Gupta, G.,Kim, C.Y.,Terwilliger, T.C. (deposition date: 2013-04-17, release date: 2013-07-03, Last modification date: 2024-02-28)
Primary citation	Hung, L.W.,Kim, H.B.,Murakami, S.,Gupta, G.,Kim, C.Y.,Terwilliger, T.C. Crystal structure of AcrB complexed with linezolid at 3.5 Angstrom resolution. J.Struct.Funct.Genom., 14:71-75, 2013 Cited by PubMed Abstract: AcrB is an inner membrane resistance-nodulation-cell division efflux pump and is part of the AcrAB-TolC tripartite efflux system. We have determined the crystal structure of AcrB with bound Linezolid at a resolution of 3.5 Å. The structure shows that Linezolid binds to the A385/F386 loops of the symmetric trimer of AcrB. A conformational change of a loop in the bottom of the periplasmic cleft is also observed. PubMed: 23673416 DOI: 10.1007/s10969-013-9154-x PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (3.5 Å)

Structure validation