4JZC

Angiopoietin-2 fibrinogen domain TAG mutant

4JZC の概要

• エントリーDOI: 10.2210/pdb4jzc/pdb
• 関連するPDBエントリー: 1Z3S 1Z3U 2GY5
• 分子名称: Angiopoietin-2 (2 entities in total)
• 機能のキーワード: fibrinogen-like receptor-binding domain, tie2 receptor-binding, tie2, signaling protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: O15123
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24817.60

構造登録者

Yu, X.,Seegar, T.C.M.,Dalton, A.C.,Tzvetkova-Robev, D.,Goldgur, Y.,Nikolov, D.B.,Barton, W.A. (登録日: 2013-04-02, 公開日: 2013-05-08, 最終更新日: 2024-11-20)

主引用文献

Yu, X.,Seegar, T.C.,Dalton, A.C.,Tzvetkova-Robev, D.,Goldgur, Y.,Rajashankar, K.R.,Nikolov, D.B.,Barton, W.A.
Structural basis for angiopoietin-1-mediated signaling initiation.
Proc.Natl.Acad.Sci.USA, 110:7205-7210, 2013

PubMed Abstract: Angiogenesis is a complex cellular process involving multiple regulatory growth factors and growth factor receptors. Among them, the ligands for the endothelial-specific tunica intima endothelial receptor tyrosine kinase 2 (Tie2) receptor kinase, angiopoietin-1 (Ang1) and Ang2, play essential roles in balancing vessel stability and regression during both developmental and tumor-induced angiogenesis. Despite possessing a high degree of sequence identity, Ang1 and Ang2 have distinct functional roles and cell-signaling characteristics. Here, we present the crystal structures of Ang1 both unbound and in complex with the Tie2 ectodomain. Comparison of the Ang1-containing structures with their Ang2-containing counterparts provide insight into the mechanism of receptor activation and reveal molecular surfaces important for interactions with Tie2 coreceptors and associated signaling proteins. Using structure-based mutagenesis, we identify a loop within the angiopoietin P domain, adjacent to the receptor-binding interface, which confers the specific agonist/antagonist properties of the molecule. We demonstrate using cell-based assays that an Ang2 chimera containing the Ang1 loop sequence behaves functionally similarly to Ang1 as a constitutive Tie2 agonist, able to efficiently dissociate the inhibitory Tie1/Tie2 complex and elicit Tie2 clustering and downstream signaling.

PubMed: 23592718
DOI: 10.1073/pnas.1216890110

実験手法

X-RAY DIFFRACTION (1.9 Å)