4JZC

Angiopoietin-2 fibrinogen domain TAG mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-02-04
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.98
Spacegroup name	I 2 2 2
Unit cell lengths	46.692, 79.055, 135.544
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.900
Rwork	0.230
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Ang-2 fibrinogen-like domain
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.600	1.600
Number of reflections	27572
Completeness [%]	92.4	92.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1.0 M Na/K-tartate, 0.1 M Tris, 0.2 M LiSO4, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K