4JRT
Crystal structure of an A-form RNA duplex containing three GU base pairs
Summary for 4JRT
| Entry DOI | 10.2210/pdb4jrt/pdb |
| Descriptor | RNA (5'-R(P*GP*GP*GP*UP*GP*GP*UP*GP*CP*GP*GP*G)-3'), RNA (5'-R(P*CP*CP*UP*GP*CP*AP*CP*UP*GP*CP*CP*C)-3') (3 entities in total) |
| Functional Keywords | a-form rna, ribose-zipper motif, gu base pair, rna |
| Total number of polymer chains | 2 |
| Total formula weight | 7702.67 |
| Authors | Kondo, J.,Dock-Bregeon, A.C.,Willkomm, D.K.,Hartmann, R.K.,Westhof, E. (deposition date: 2013-03-21, release date: 2013-06-05, Last modification date: 2023-11-08) |
| Primary citation | Kondo, J.,Dock-Bregeon, A.C.,Willkomm, D.K.,Hartmann, R.K.,Westhof, E. Structure of an A-form RNA duplex obtained by degradation of 6S RNA in a crystallization droplet Acta Crystallogr.,Sect.F, 69:634-639, 2013 Cited by PubMed Abstract: In the course of a crystallographic study of a 132 nt variant of Aquifex aeolicus 6S RNA, a crystal structure of an A-form RNA duplex containing 12 base pairs was solved at a resolution of 2.6 Å. In fact, the RNA duplex is part of the 6S RNA and was obtained by accidental but precise degradation of the 6S RNA in a crystallization droplet. 6S RNA degradation was confirmed by microscopic observation of crystals and gel electrophoresis of crystallization droplets. The RNA oligomers obtained form regular A-form duplexes containing three GoU wobble-type base pairs, one of which engages in intermolecular contacts through a ribose-zipper motif at the crystal-packing interface. PubMed: 23722840DOI: 10.1107/S1744309113013018 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.6 Å) |
Structure validation
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