4JRT

Crystal structure of an A-form RNA duplex containing three GU base pairs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-04
Wavelength(s)	1.0723
Spacegroup name	P 43
Unit cell lengths	29.330, 29.330, 89.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.700 - 2.600
Rwork	0.185
R-free	0.22750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	353d
RMSD bond length	0.006
RMSD bond angle	1.195
Data reduction software	CrystalClear
Data scaling software	CrystalClear
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	20.700
High resolution limit [Å]	2.600
Number of reflections	2330

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	310	Sodium Cacodylate, Lithium acetate, PEG3350, pH 7.0, vapor diffusion, temperature 310K