4JQU

Crystal structure of Ubc7p in complex with the U7BR of Cue1p

4JQU の概要

• エントリーDOI: 10.2210/pdb4jqu/pdb
• 分子名称: Ubiquitin-conjugating enzyme E2 7, Coupling of ubiquitin conjugation to ER degradation protein 1, 2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, ... (5 entities in total)
• 機能のキーワード: ubc7p:u7br complex, ligase-activator complex, ligase-protein binding complex, ligase/protein binding
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• 細胞内の位置: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side: Q02159; Endoplasmic reticulum membrane ; Single-pass membrane protein : P38428
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 25671.37

構造登録者

Liang, Y.-H.,Metzger, M.B.,Weissman, A.M.,Ji, X. (登録日: 2013-03-20, 公開日: 2013-05-29, 最終更新日: 2023-09-20)

主引用文献

Metzger, M.B.,Liang, Y.H.,Das, R.,Mariano, J.,Li, S.,Li, J.,Kostova, Z.,Byrd, R.A.,Ji, X.,Weissman, A.M.
A Structurally Unique E2-Binding Domain Activates Ubiquitination by the ERAD E2, Ubc7p, through Multiple Mechanisms.
Mol.Cell, 50:516-527, 2013

PubMed Abstract: Cue1p is an integral component of yeast endoplasmic reticulum (ER)-associated degradation (ERAD) ubiquitin ligase (E3) complexes. It tethers the ERAD ubiquitin-conjugating enzyme (E2), Ubc7p, to the ER and prevents its degradation, and also activates Ubc7p via unknown mechanisms. We have now determined the crystal structure of the Ubc7p-binding region (U7BR) of Cue1p with Ubc7p. The U7BR is a unique E2-binding domain that includes three α-helices that interact extensively with the "backside" of Ubc7p. Residues essential for E2 binding are also required for activation of Ubc7p and for ERAD. We establish that the U7BR stimulates both RING-independent and RING-dependent ubiquitin transfer from Ubc7p. Moreover, the U7BR enhances ubiquitin-activating enzyme (E1)-mediated charging of Ubc7p with ubiquitin. This demonstrates that an essential component of E3 complexes can simultaneously bind to E2 and enhance its loading with ubiquitin. These findings provide mechanistic insights into how ubiquitination can be stimulated.

PubMed: 23665230
DOI: 10.1016/j.molcel.2013.04.004

実験手法

X-RAY DIFFRACTION (1.81 Å)