4JIO

Bro1 V domain and ubiquitin

4JIO の概要

• エントリーDOI: 10.2210/pdb4jio/pdb
• 分子名称: BRO1, Ubiquitin (2 entities in total)
• 機能のキーワード: membrane trafficking, escrt-i, escrt-iii, ubiquitin, endosome, protein binding
• 由来する生物種: Naumovozyma castellii (Yeast); 詳細
• 細胞内の位置: Ubiquitin: Cytoplasm (By similarity). 60S ribosomal protein L40: Cytoplasm (By similarity): P62987
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 87862.52

構造登録者

Pashkova, N.,Gakhar, L.,Piper, R.C. (登録日: 2013-03-06, 公開日: 2013-06-19, 最終更新日: 2024-11-20)

主引用文献

Pashkova, N.,Gakhar, L.,Winistorfer, S.C.,Sunshine, A.B.,Rich, M.,Dunham, M.J.,Yu, L.,Piper, R.C.
The yeast alix homolog bro1 functions as a ubiquitin receptor for protein sorting into multivesicular endosomes.
Dev.Cell, 25:520-533, 2013

PubMed Abstract: Sorting of ubiquitinated membrane proteins into lumenal vesicles of multivesicular bodies is mediated by the Endosomal Sorting Complex Required for Transport (ESCRT) apparatus and accessory proteins such as Bro1, which recruits the deubiquitinating enzyme Doa4 to remove ubiquitin from cargo. Here we propose that Bro1 works as a receptor for the selective sorting of ubiquitinated cargoes. We found synthetic genetic interactions between BRO1 and ESCRT-0, suggesting that Bro1 functions similarly to ESCRT-0. Multiple structural approaches demonstrated that Bro1 binds ubiquitin via the N-terminal trihelical arm of its middle V domain. Mutants of Bro1 that lack the ability to bind Ub were dramatically impaired in their ability to sort Ub-cargo membrane proteins, but only when combined with hypomorphic alleles of ESCRT-0. These data suggest that Bro1 and other Bro1 family members function in parallel with ESCRT-0 to recognize and sort Ub-cargoes.

PubMed: 23726974
DOI: 10.1016/j.devcel.2013.04.007

実験手法

X-RAY DIFFRACTION (3.6 Å)