4JH9

Crystal Structure of FosB from Bacillus cereus with Maganese and Potential BSH-Fosfomycin Product

Summary for 4JH9

• Entry DOI: 10.2210/pdb4jh9/pdb
• Related: 4JH1 4JH2 4JH3 4JH4 4JH5 4JH6 4JH7 4JH8
• Descriptor: Metallothiol transferase FosB, MANGANESE (II) ION, (2R)-2-azanyl-3-[(1R,2S)-2-oxidanyl-1-phosphono-propyl]sulfanyl-propanoic acid, ... (4 entities in total)
• Functional Keywords: bacillithiol-s-transferase, transferase-transferase inhibitor complex, transferase/transferase inhibitor
• Biological source: Bacillus cereus
• Cellular location: Cytoplasm : Q739M9
• Total number of polymer chains: 2
• Total formula weight: 33605.28

Authors

Thompson, M.K.,Harp, J.,Keithly, M.E.,Jagessar, K.,Cook, P.D.,Armstrong, R.N. (deposition date: 2013-03-04, release date: 2013-10-02, Last modification date: 2024-02-28)

Primary citation

Thompson, M.K.,Keithly, M.E.,Harp, J.,Cook, P.D.,Jagessar, K.L.,Sulikowski, G.A.,Armstrong, R.N.
Structural and Chemical Aspects of Resistance to the Antibiotic Fosfomycin Conferred by FosB from Bacillus cereus.
Biochemistry, 52:7350-7362, 2013

PubMed Abstract: The fosfomycin resistance enzymes, FosB, from Gram-positive organisms, are M(2+)-dependent thiol tranferases that catalyze nucleophilic addition of either L-cysteine (L-Cys) or bacillithiol (BSH) to the antibiotic, resulting in a modified compound with no bacteriacidal properties. Here we report the structural and functional characterization of FosB from Bacillus cereus (FosB(Bc)). The overall structure of FosB(Bc), at 1.27 Å resolution, reveals that the enzyme belongs to the vicinal oxygen chelate (VOC) superfamily. Crystal structures of FosB(Bc) cocrystallized with fosfomycin and a variety of divalent metals, including Ni(2+), Mn(2+), Co(2+), and Zn(2+), indicate that the antibiotic coordinates to the active site metal center in an orientation similar to that found in the structurally homologous manganese-dependent fosfomycin resistance enzyme, FosA. Surface analysis of the FosB(Bc) structures show a well-defined binding pocket and an access channel to C1 of fosfomycin, the carbon to which nucleophilic addition of the thiol occurs. The pocket and access channel are appropriate in size and shape to accommodate L-Cys or BSH. Further investigation of the structures revealed that the fosfomycin molecule, anchored by the metal, is surrounded by a cage of amino acids that hold the antibiotic in an orientation such that C1 is centered at the end of the solvent channel, positioning the compound for direct nucleophilic attack by the thiol substrate. In addition, the structures of FosB(Bc) in complex with the L-Cys-fosfomycin product (1.55 Å resolution) and in complex with the bacillithiol-fosfomycin product (1.77 Å resolution) coordinated to a Mn(2+) metal in the active site have been determined. The L-Cys moiety of either product is located in the solvent channel, where the thiol has added to the backside of fosfomycin C1 located at the end of the channel. Concomitant kinetic analyses of FosB(Bc) indicated that the enzyme has a preference for BSH over L-Cys when activated by Mn(2+) and is inhibited by Zn(2+). The fact that Zn(2+) is an inhibitor of FosB(Bc) was used to obtain a ternary complex structure of the enzyme with both fosfomycin and L-Cys bound.

PubMed: 24004181
DOI: 10.1021/bi4009648

Experimental method

X-RAY DIFFRACTION (1.77 Å)