4JGF

Crystal Structure of the Cataract-Causing P23T gamma D-Crystallin Mutant

Summary for 4JGF

• Entry DOI: 10.2210/pdb4jgf/pdb
• Descriptor: Gamma-crystallin D (2 entities in total)
• Functional Keywords: structural protein
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 2
• Total formula weight: 40809.41

Authors

Ji, F.L.,Koharudin, L.M.,Jung, J.W.,Gronenborn, A.M. (deposition date: 2013-03-01, release date: 2013-05-22, Last modification date: 2024-02-28)

Primary citation

Ji, F.,Koharudin, L.M.,Jung, J.,Gronenborn, A.M.
Crystal structure of the cataract-causing P23T gamma D-crystallin mutant.
Proteins, 81:1493-1498, 2013

PubMed Abstract: Up to now, efforts to crystallize the cataract-associated P23T mutant of human γD-crystallin have not been successful. Therefore, insights into the light scattering mechanism of this mutant have been exclusively obtained from solution work. Here we present the first crystal structure of the P23T mutant at 2.5 Å resolution. The protein exhibits essentially the same overall structure as seen for the wild-type protein. Based on our structural data, we confirm that no major conformational changes are caused by the mutation, and that solution phase properties of the mutant appear exclusively associated with cataract formation.

PubMed: 23670788
DOI: 10.1002/prot.24321

Experimental method

X-RAY DIFFRACTION (2.5 Å)