4JGF
Crystal Structure of the Cataract-Causing P23T gamma D-Crystallin Mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2012-09-04 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.550, 101.931, 41.371 |
Unit cell angles | 90.00, 96.54, 90.00 |
Refinement procedure
Resolution | 32.010 - 2.500 |
R-factor | 0.23349 |
Rwork | 0.228 |
R-free | 0.28352 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.871 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.010 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 10719 | |
Completeness [%] | 100.0 | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 0.17 M CH3COONH4, 0.085 M C2H3NaO2 3H2O, 25.5% (w/v) PEG 4000, and 15% (v/v) glycerol , pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |