4JFA

Crystal Structure of Plasmodium falciparum Tryptophanyl-tRNA synthetase

Summary for 4JFA

• Entry DOI: 10.2210/pdb4jfa/pdb
• Related: 2AZX 2QUH
• Descriptor: Tryptophan--tRNA ligase, TRYPTOPHAN, BETA-MERCAPTOETHANOL, ... (5 entities in total)
• Functional Keywords: wrs, trprs, plasmodium, ligase
• Biological source: Plasmodium falciparum
• Total number of polymer chains: 4
• Total formula weight: 196439.42

Authors

Khan, S.,Garg, A.,Manickam, Y.,Sharma, A. (deposition date: 2013-02-28, release date: 2014-01-29, Last modification date: 2025-03-26)

Primary citation

Khan, S.,Garg, A.,Sharma, A.,Camacho, N.,Picchioni, D.,Saint-Leger, A.,Ribas de Pouplana, L.,Yogavel, M.,Sharma, A.
An appended domain results in an unusual architecture for malaria parasite tryptophanyl-tRNA synthetase
Plos One, 8:e66224-e66224, 2013

PubMed Abstract: Specific activation of amino acids by aminoacyl-tRNA synthetases (aaRSs) is essential for maintaining fidelity during protein translation. Here, we present crystal structure of malaria parasite Plasmodium falciparum tryptophanyl-tRNA synthetase (Pf-WRS) catalytic domain (AAD) at 2.6 Å resolution in complex with L-tryptophan. Confocal microscopy-based localization data suggest cytoplasmic residency of this protein. Pf-WRS has an unusual N-terminal extension of AlaX-like domain (AXD) along with linker regions which together seem vital for enzymatic activity and tRNA binding. Pf-WRS is not proteolytically processed in the parasites and therefore AXD likely provides tRNA binding capability rather than editing activity. The N-terminal domain containing AXD and linker region is monomeric and would result in an unusual overall architecture for Pf-WRS where the dimeric catalytic domains have monomeric AXDs on either side. Our PDB-wide comparative analyses of 47 WRS crystal structures also provide new mechanistic insights into this enzyme family in context conserved KMSKS loop conformations.

PubMed: 23776638
DOI: 10.1371/journal.pone.0066224

Experimental method

X-RAY DIFFRACTION (2.6 Å)