4JAG

STRUCTURAL DETERMINATION OF THE A50T:S279G:S280K:V281K:K282E:H283N VARIANT OF CITRATE SYNTHASE FROM E. COLI COMPLEXED WITH oxaloacetate

Replaces:  3L99

Summary for 4JAG

• Entry DOI: 10.2210/pdb4jag/pdb
• Related: 4G6B 4JAD 4JAE 4JAF
• Descriptor: Citrate synthase, OXALOACETATE ION, SULFATE ION, ... (4 entities in total)
• Functional Keywords: citrate synthase, gram-negative bacteria, allostery, oxaloacetate, acetyl-coa, nadh, protein folding, s-carboxymethyl-coa, allosteric enzyme, transferase, tricarboxylic acid cycle
• Biological source: Escherichia coli
• Total number of polymer chains: 2
• Total formula weight: 96635.95

Authors

Maurus, R.,Brayer, G.D. (deposition date: 2013-02-18, release date: 2013-07-17, Last modification date: 2023-09-20)

• Primary citation: Duckworth, H.W.,Nguyen, N.T.,Gao, Y.,Donald, L.J.,Maurus, R.,Ayed, A.,Bruneau, B.,Brayer, G.D.; Enzyme-substrate complexes of allosteric citrate synthase: Evidence for a novel intermediate in substrate binding.; Biochim.Biophys.Acta, 1834:2546-2553, 2013; PubMed: 23954305; DOI: 10.1016/j.bbapap.2013.07.019

Experimental method

X-RAY DIFFRACTION (2.1 Å)