4J4J

Crystal structure of the APOBEC3F Vif binding domain

4J4J の概要

• エントリーDOI: 10.2210/pdb4j4j/pdb
• 分子名称: DNA dC->dU-editing enzyme APOBEC-3F, ZINC ION (2 entities in total)
• 機能のキーワード: alpha/beta, hydrolase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: Q8IUX4
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 49970.34

構造登録者

Siu, K.K.,Sultana, A.,Lee, J.E. (登録日: 2013-02-06, 公開日: 2013-11-06, 最終更新日: 2024-02-28)

主引用文献

Siu, K.K.,Sultana, A.,Azimi, F.C.,Lee, J.E.
Structural determinants of HIV-1 Vif susceptibility and DNA binding in APOBEC3F.
Nat Commun, 4:2593-2593, 2013

PubMed Abstract: The human APOBEC3 family of DNA cytosine deaminases serves as a front-line intrinsic immune response to inhibit the replication of diverse retroviruses. APOBEC3F and APOBEC3G are the most potent factors against HIV-1. As a countermeasure, HIV-1 viral infectivity factor (Vif) targets APOBEC3s for proteasomal degradation. Here we report the crystal structure of the Vif-binding domain in APOBEC3F and a novel assay to assess Vif-APOBEC3 binding. Our results point to an amphipathic surface that is conserved in APOBEC3s as critical for Vif susceptibility in APOBEC3F. Electrostatic interactions likely mediate Vif binding. Moreover, structure-guided mutagenesis reveals a straight ssDNA-binding groove distinct from the Vif-binding site, and an 'aromatic switch' is proposed to explain DNA substrate specificities across the APOBEC3 family. This study opens new lines of inquiry that will further our understanding of APOBEC3-mediated retroviral restriction and provides an accurate template for structure-guided development of inhibitors targeting the APOBEC3-Vif axis.

PubMed: 24185281
DOI: 10.1038/ncomms3593

実験手法

X-RAY DIFFRACTION (3.1 Å)