4J0W

Structure of U3-55K

Summary for 4J0W

• Entry DOI: 10.2210/pdb4j0w/pdb
• Related: 4J0X
• Descriptor: U3 small nucleolar RNA-interacting protein 2 (2 entities in total)
• Functional Keywords: beta-propeller, wd domain, rna binding protein, preribosome
• Biological source: Homo sapiens (human)
• Cellular location: Nucleus, nucleolus: O43818
• Total number of polymer chains: 1
• Total formula weight: 37443.61

Authors

Zhang, L.,Lin, J.,Ye, K. (deposition date: 2013-01-31, release date: 2013-06-05, Last modification date: 2023-11-08)

Primary citation

Zhang, L.,Lin, J.,Ye, K.
Structural and functional analysis of the U3 snoRNA binding protein Rrp9.
Rna, 19:701-711, 2013

PubMed Abstract: The U3 snoRNA is required for 18S rRNA processing and small subunit ribosome formation in eukaryotes. Different from other box C/D snoRNAs, U3 contains an extra 5' domain that pairs with pre-rRNA and a unique B/C motif essential for recruitment of the U3-specific Rrp9 protein. Here, we analyze the structure and function of Rrp9 with crystallographic, biochemical, and cellular approaches. Rrp9 is composed of a WD repeat domain and an N-terminal region. The crystal structures of the WD domain of yeast Rrp9 and its human ortholog U3-55K were determined, revealing a typical seven-bladed propeller fold. Several conserved surface patches on the WD domain were identified, and their function in RNP assembly and yeast growth were analyzed by mutagenesis. Prior association of Snu13 with the B/C motif was found to enhance the specific binding of the WD domain. We show that a conserved 7bc loop is crucial for specific recognition of U3, nucleolar localization of Rrp9, and yeast growth. In addition, we show that the N-terminal region of Rrp9 contains a bipartite nuclear localization signal that is dispensable for nucleolar localization. Our results provide insight into the functional sites of Rrp9.

PubMed: 23509373
DOI: 10.1261/rna.037580.112

Experimental method

X-RAY DIFFRACTION (1.7 Å)