4J0E

Crystal structure of 3-hydroxyacyl-CoA dehydrogenase from Caenorhadbitis elegans in P1 space group

4J0E の概要

• エントリーDOI: 10.2210/pdb4j0e/pdb
• 関連するPDBエントリー: 4J0F
• 分子名称: Probable 3-hydroxyacyl-CoA dehydrogenase F54C8.1 (2 entities in total)
• 機能のキーワード: rossmann fold, dehydrogenase, fatty acid beta-oxidation, nadh binding, mitochondrial matrix, oxidoreductase
• 由来する生物種: Caenorhabditis elegans (nematode)
• 細胞内の位置: Mitochondrion matrix : P34439
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 69419.45

構造登録者

Xu, Y.,Sun, F.,Zhai, Y. (登録日: 2013-01-30, 公開日: 2014-03-19, 最終更新日: 2023-11-08)

主引用文献

Xu, Y.,Sun, F.
Purification, crystallization and preliminary crystallographic analysis of 3-hydroxyacyl-CoA dehydrogenase from Caenorhabditis elegans.
Acta Crystallogr.,Sect.F, 69:515-519, 2013

PubMed Abstract: 3-Hydroxyacyl-CoA dehydrogenase (HAD; EC 1.1.1.35) is the enzyme that catalyzes the third step in fatty-acid β-oxidation, oxidizing the hydroxyl group of 3-hydroxyacyl-CoA to a keto group. The 3-hydroxyacyl-CoA dehydrogenase from Caenorhabditis elegans (cHAD) was cloned, overexpressed in Escherichia coli and purified to homogeneity for crystallography. Initial crystals were obtained by the hanging-drop vapour-diffusion method. Optimization of the precipitant concentration and the pH yielded two types of well diffracting crystals with parallelepiped and cuboid shapes, respectively. Complete diffraction data sets were collected and processed from both crystal types. Preliminary crystallographic analysis indicated that the parallelepiped-shaped crystal belonged to space group P1, while the cuboid-shaped crystal belonged to space group P212121. Analyses of computed Matthews coefficient and self-rotation functions suggested that there are two cHAD molecules in one asymmetric unit in both crystals, forming identical dimers but packing in distinct manners.

PubMed: 23695566
DOI: 10.1107/S1744309113007045

実験手法

X-RAY DIFFRACTION (1.6 Å)