4IWW

Computational Design of an Unnatural Amino Acid Metalloprotein with Atomic Level Accuracy

Summary for 4IWW

• Entry DOI: 10.2210/pdb4iww/pdb
• Related: 1IGS 4IX0
• Descriptor: Unnatural Amino Acid Mediated Metalloprotein, SULFATE ION, COBALT (II) ION, ... (4 entities in total)
• Functional Keywords: computational design, tim barrel, metalloprotein, bipyridylalanine, metal binding protein
• Biological source: Sulfolobus solfataricus
• Total number of polymer chains: 2
• Total formula weight: 60530.61

Authors

Mills, J.,Bolduc, J.,Khare, S.,Stoddard, B.,Baker, D. (deposition date: 2013-01-24, release date: 2013-08-21, Last modification date: 2023-11-15)

Primary citation

Mills, J.H.,Khare, S.D.,Bolduc, J.M.,Forouhar, F.,Mulligan, V.K.,Lew, S.,Seetharaman, J.,Tong, L.,Stoddard, B.L.,Baker, D.
Computational design of an unnatural amino Acid dependent metalloprotein with atomic level accuracy.
J.Am.Chem.Soc., 135:13393-13399, 2013

PubMed Abstract: Genetically encoded unnatural amino acids could facilitate the design of proteins and enzymes of novel function, but correctly specifying sites of incorporation and the identities and orientations of surrounding residues represents a formidable challenge. Computational design methods have been used to identify optimal locations for functional sites in proteins and design the surrounding residues but have not incorporated unnatural amino acids in this process. We extended the Rosetta design methodology to design metalloproteins in which the amino acid (2,2'-bipyridin-5yl)alanine (Bpy-Ala) is a primary ligand of a bound metal ion. Following initial results that indicated the importance of buttressing the Bpy-Ala amino acid, we designed a buried metal binding site with octahedral coordination geometry consisting of Bpy-Ala, two protein-based metal ligands, and two metal-bound water molecules. Experimental characterization revealed a Bpy-Ala-mediated metalloprotein with the ability to bind divalent cations including Co(2+), Zn(2+), Fe(2+), and Ni(2+), with a Kd for Zn(2+) of ∼40 pM. X-ray crystal structures of the designed protein bound to Co(2+) and Ni(2+) have RMSDs to the design model of 0.9 and 1.0 Å respectively over all atoms in the binding site.

PubMed: 23924187
DOI: 10.1021/ja403503m

Experimental method

X-RAY DIFFRACTION (2.3 Å)