4ITP

Structure of human carbonic anhydrase II bound to a benzene sulfonamide

4ITP の概要

• エントリーDOI: 10.2210/pdb4itp/pdb
• 関連するPDBエントリー: 4ITO
• 分子名称: Carbonic anhydrase 2, GLYCEROL, ZINC ION, ... (6 entities in total)
• 機能のキーワード: alpha beta fold, reversible hydration of carbon dioxide to bicarbonate and proton, lyase-lyase inhibitor complex, lyase/lyase inhibitor
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: P00918
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29841.02

構造登録者

Biswas, S.,McKenna, R. (登録日: 2013-01-18, 公開日: 2014-01-01, 最終更新日: 2024-02-28)

主引用文献

Guzel-Akdemir, O.,Biswas, S.,Lastra, K.,McKenna, R.,Supuran, C.T.
Structural study of the location of the phenyl tail of benzene sulfonamides and the effect on human carbonic anhydrase inhibition.
Bioorg.Med.Chem., 21:6674-6680, 2013

PubMed Abstract: The crystal structure of 4-phenylacetamidomethyl-benzenesulfonamide (4ITP) bound to human carbonic anhydrase (hCA, EC 4.2.1.1) II is reported. 4ITP is a medium potency hCA I and II inhibitor (KIs of 54-75nM), a strong mitochondrial CA VA/VB inhibitor (KIs of 8.3-8.6nM) and a weak transmembrane CA inhibitor (KIs of 136-212nM against hCA IX and XII). This elongated compound binds in an extended conformation to hCA II, with its tail lying towards the hydrophobic half of the active site whereas the sulfonamide moiety coordinates the zinc ion. The present structure was compared to that of structurally related aromatic sulfonamides, such as 4-phenylacetamido-benzene-sulfonamide (3OYS), 4-(2-mercaptophenylacetamido)-benzene-sulfonamide (2HD6) and 4-(3-nitrophenyl)-ureido-benzenesulfonamide (3N2P). Homology models of the hCA I, VA, VB, IX and XII structures were build which afforded an understanding of the amino acids involved in the binding of these compounds to these isoforms. The main conclusion of the study is that the orientation of the tail moiety and the presence of flexible linkers as well polar groups in it, strongly influence the potency and the selectivity of the sulfonamides for the inhibition of cytosolic, mitochondrial or transmembrane CA isoforms.

PubMed: 24012377
DOI: 10.1016/j.bmc.2013.08.011

実験手法

X-RAY DIFFRACTION (1.697 Å)