4IS5

Crystal Structure of the ligand-free inactive Matriptase

4IS5 の概要

• エントリーDOI: 10.2210/pdb4is5/pdb
• 関連するPDBエントリー: 4ISL 4ISN 4ISO
• 分子名称: Suppressor of tumorigenicity 14 protein, SULFATE ION, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: beta barrel, serine protease, epithelium, hydrolase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Membrane ; Single-pass type II membrane protein : Q9Y5Y6
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27429.64

構造登録者

Huang, M.D.,Zhao, B.Y.,Yuan, C.,Li, R. (登録日: 2013-01-16, 公開日: 2013-03-06, 最終更新日: 2023-09-20)

主引用文献

Zhao, B.,Yuan, C.,Li, R.,Qu, D.,Huang, M.,Ngo, J.C.
Crystal structures of matriptase in complex with its inhibitor hepatocyte growth factor activator inhibitor-1.
J.Biol.Chem., 288:11155-11164, 2013

PubMed Abstract: Matriptase, a type II trans-membrane serine protease of the S1 trypsin-like family, is expressed on the surface of nearly all normal human epithelium and found in biological fluid-like human milk. Matriptase overexpression has been implicated in tumor progression in certain epithelium-derived cancer cells. Matriptase is tightly regulated by its cognate inhibitor hepatocyte growth factor activator inhibitor-1 (HAI-1). It has been demonstrated that the Kunitz domain I (KD1) but not Kunitz domain II (KD2) of HAI-1 is responsible for the inhibitory activity of HAI-1 against matriptase. To investigate the molecular basis of inhibition of matriptase by HAI-1, we solved several crystal structures of matriptase serine protease domain in complex with the fragments of HAI-1. Based on these structures, we found that the binding of KD1 was different from previously predicted binding mode. The P3 arginine residue occupies the S3 specificity pocket of matriptase, but not the S4 pocket as in the cases of hepatocyte growth factor activator·HAI-1 KD1 and matriptase·sunflower trypsin inhibitor-1 complexes. The long 60-loop of matriptase makes direct contact with HAI-1 but remains flexible even in the complexes, and its apex does not bind with KD1 tightly. The interactions between this unique 60-loop and KD1 may provide an opportunity to increase the specificity and inhibitory activity of KD1 for matriptase. Furthermore, comparison between KD1 and a homology model of HAI-1 KD2 rationalizes the structural basis of why KD1 but not KD2 is responsible for the inhibitory activity of HAI-1 against matriptase.

PubMed: 23443661
DOI: 10.1074/jbc.M113.454611

実験手法

X-RAY DIFFRACTION (1.48 Å)