4IRH

Auto-inhibited ERG Ets domain

4IRH の概要

• エントリーDOI: 10.2210/pdb4irh/pdb
• 関連するPDBエントリー: 4IRG 4IRI
• 分子名称: Transcriptional regulator ERG (2 entities in total)
• 機能のキーワード: winged helix-turn-helix, ets domain, dna binding protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: P11308
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15059.30

構造登録者

Regan, M.C.,Horanyi, P.S.,Pryor, E.E.,Sarver, J.L.,Cafiso, D.S.,Bushweller, J.H. (登録日: 2013-01-14, 公開日: 2013-07-31, 最終更新日: 2024-10-09)

主引用文献

Regan, M.C.,Horanyi, P.S.,Pryor, E.E.,Sarver, J.L.,Cafiso, D.S.,Bushweller, J.H.
Structural and dynamic studies of the transcription factor ERG reveal DNA binding is allosterically autoinhibited.
Proc.Natl.Acad.Sci.USA, 110:13374-13379, 2013

PubMed Abstract: The Ets-Related Gene (ERG) belongs to the Ets family of transcription factors and is critically important for maintenance of the hematopoietic stem cell population. A chromosomal translocation observed in the majority of human prostate cancers leads to the aberrant overexpression of ERG. We have identified regions flanking the ERG Ets domain responsible for autoinhibition of DNA binding and solved crystal structures of uninhibited, autoinhibited, and DNA-bound ERG. NMR-based measurements of backbone dynamics show that uninhibited ERG undergoes substantial dynamics on the millisecond-to-microsecond timescale but autoinhibited and DNA-bound ERG do not. We propose a mechanism whereby the allosteric basis of ERG autoinhibition is mediated predominantly by the regulation of Ets-domain dynamics with only modest structural changes.

PubMed: 23898196
DOI: 10.1073/pnas.1301726110

実験手法

X-RAY DIFFRACTION (2.1 Å)