4IQJ

Structure of PolIIIalpha-Tauc-DNA complex suggests an atomic model of the replisome

4IQJ の概要

• エントリーDOI: 10.2210/pdb4iqj/pdb
• 関連するPDBエントリー: 2HPI 3E0D
• 分子名称: DNA (5'-D(P*CP*GP*AP*AP*AP*CP*GP*AP*CP*GP*GP*CP*CP*AP*GP*TP*GP*CP*CP*A)-3'), DNA (5'-D(*TP*TP*TP*TP*TP*TP*TP*GP*TP*GP*GP*CP*AP*CP*TP*GP*GP*CP*CP*GP*TP*CP*GP*TP*TP*TP*CP*G)-3'), DNA (5'-D(P*CP*GP*AP*AP*AP*CP*GP*AP*CP*GP*GP*CP*CP*AP*GP*TP*GP*CP*CP*AP*(DOC))-3'), ... (7 entities in total)
• 機能のキーワード: polymerase, alpha subunit, tauc subunit, dna replication, dna-directed dna polymerase, nucleotidyltransferase, transferase-dna complex, transferase/dna
• 由来する生物種: Thermus aquaticus; 詳細
• 細胞内の位置: Cytoplasm (By similarity): 4IQJ
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 688792.08

構造登録者

Liu, B.,Lin, J.,Steitz, T. (登録日: 2013-01-11, 公開日: 2013-03-13, 最終更新日: 2023-09-20)

主引用文献

Liu, B.,Lin, J.,Steitz, T.A.
Structure of PolIIIalpha-Tauc-DNA complex suggests an atomic model of the replisome
Structure, 21:658-664, 2013

PubMed Abstract: The C-terminal domain (CTD) of the τ subunit of the clamp loader (τc) binds to both the DnaB helicase and the DNA polymerase III α subunit (PolIIIα), and determines their relative positions and orientations on the leading and lagging strands. Here, we present a 3.2 Å resolution structure of Thermus aquaticus PolIIIα in complex with τc and a DNA substrate. The structure reveals that the CTD of τc interacts with the CTD of PolIIIα through its C-terminal helix and the adjacent loop. Additionally, in this complex PolIIIα displays an open conformation that includes the reorientations of the oligonucleotide-binding fold and the thumb domain, which may be an indirect result of crystal packing due to the presence of the τc. Nevertheless, the position of the τc on PolIIIα allows us to suggest an approximate model for how the PolIIIα is oriented and positioned on the DnaB helicase.

PubMed: 23478062
DOI: 10.1016/j.str.2013.02.002

実験手法

X-RAY DIFFRACTION (3.2 Å)