4ILM

CRISPR RNA Processing endoribonuclease

4ILM の概要

• エントリーDOI: 10.2210/pdb4ilm/pdb
• 関連するPDBエントリー: 3PKM 4ILL 4ILR
• 分子名称: CRISPR-associated endoribonuclease Cas6 2, RNA (5'-R(*GP*CP*UP*AP*AP*UP*CP*UP*AP*CP*UP*AP*UP*AP*GP*A)-3') (2 entities in total)
• 機能のキーワード: rrm, endoribonuclease, rna, hydrolase-rna complex, hydrolase/rna
• 由来する生物種: Sulfolobus solfataricus; 詳細
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 302009.65

構造登録者

Shao, Y.,Li, H. (登録日: 2012-12-31, 公開日: 2013-03-20, 最終更新日: 2024-02-28)

主引用文献

Shao, Y.,Li, H.
Recognition and cleavage of a nonstructured CRISPR RNA by its processing endoribonuclease Cas6.
Structure, 21:385-393, 2013

PubMed Abstract: Clustered regularly interspaced short palindromic repeats (CRISPRs) confer adaptive immunity to prokaryotes through a small RNA-mediated mechanism. Specific endoribonucleases are required by all CRISPR-bearing organisms to process CRISPR RNAs into small RNA that serve as guides for defensive effector complexes. The molecular mechanism of how the endoribonucleases process the class of CRISPR RNA containing no predicted secondary structural features remains largely elusive. Here, we report cocrystal structures of a processing endoribonuclease bound with a noncleavable RNA substrate and its product-like fragment derived from a nonpalindramic repeat. The enzyme stabilizes a short RNA stem-loop structure near the cleavage site and cleaves the phosphodiester bond using an active site comprised of arginine and lysine residues. The distinct RNA binding and cleavage mechanisms underline the diversity in CRISPR RNA processing.

PubMed: 23454186
DOI: 10.1016/j.str.2013.01.010

実験手法

X-RAY DIFFRACTION (3.068 Å)