4ILM
CRISPR RNA Processing endoribonuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-04-21 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.981, 154.608, 130.848 |
| Unit cell angles | 90.00, 93.64, 90.00 |
Refinement procedure
| Resolution | 35.000 - 3.068 |
| R-factor | 0.2346 |
| Rwork | 0.233 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.229 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 57752 | |
| <I/σ(I)> | 24.9 | |
| Completeness [%] | 95.9 | 70.6 |
| Redundancy | 6.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 303 | 15%(v/v) isopropanol, 1mM spermine, 10mM Co(NH4)6Cl3, 50mM Tris-HCl (pH 7.0), VAPOR DIFFUSION, HANGING DROP, temperature 303K |
