4I6Y

3-hydroxy-3-methyl (HMG) Coenzyme A Reductase bound to R-Mevalonate

Summary for 4I6Y

• Entry DOI: 10.2210/pdb4i6y/pdb
• Related: 4I4B 4I56 4I64 4I6A 4I6W
• Descriptor: 3-hydroxy-3-methylglutaryl-coenzyme A reductase, (R)-MEVALONATE, GLYCEROL, ... (6 entities in total)
• Functional Keywords: oxidoreductase
• Biological source: Pseudomonas mevalonii
• Total number of polymer chains: 2
• Total formula weight: 92567.54

Authors

Steussy, C.N.,Stauffacher, C.V.,Schmidt, T.,Burgner II, J.W.,Rodwell, V.W.,Wrensford, L.V.,Critchelow, C.J.,Min, J. (deposition date: 2012-11-30, release date: 2013-07-17, Last modification date: 2024-11-27)

Primary citation

Steussy, C.N.,Critchelow, C.J.,Schmidt, T.,Min, J.K.,Wrensford, L.V.,Burgner, J.W.,Rodwell, V.W.,Stauffacher, C.V.
A Novel Role for Coenzyme A during Hydride Transfer in 3-Hydroxy-3-methylglutaryl-coenzyme A Reductase.
Biochemistry, 52:5195-5205, 2013

PubMed Abstract: In this study, we take advantage of the ability of HMG-CoA reductase (HMGR) from Pseudomonas mevalonii to remain active while in its crystallized form to study the changing interactions between the ligands and protein as the first reaction intermediate is created. HMG-CoA reductase catalyzes one of the few double oxidation-reduction reactions in intermediary metabolism that take place in a single active site. Our laboratory has undertaken an exploration of this reaction space using structures of HMG-CoA reductase complexed with various substrate, nucleotide, product, and inhibitor combinations. With a focus in this publication on the first hydride transfer, our structures follow this reduction reaction as the enzyme converts the HMG-CoA thioester from a flat sp(2)-like geometry to a pyramidal thiohemiacetal configuration consistent with a transition to an sp(3) orbital. This change in the geometry propagates through the coenzyme A (CoA) ligand whose first amide bond is rotated 180° where it anchors a web of hydrogen bonds that weave together the nucleotide, the reaction intermediate, the enzyme, and the catalytic residues. This creates a stable intermediate structure prepared for nucleotide exchange and the second reduction reaction within the HMG-CoA reductase active site. Identification of this reaction intermediate provides a template for the development of an inhibitor that would act as an antibiotic effective against the HMG-CoA reductase of methicillin-resistant Staphylococcus aureus.

PubMed: 23802607
DOI: 10.1021/bi400335g

Experimental method

X-RAY DIFFRACTION (1.45 Å)