4I0K

Crystal structure of murine B7-H3 extracellular domain

Summary for 4I0K

• Entry DOI: 10.2210/pdb4i0k/pdb
• Descriptor: CD276 antigen, alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• Functional Keywords: immunoglobulin domain, glycoprotein, immunity, adaptive immunity, structural genomics, protein structure initiative, immunoglobulin variable like domain, immunoglobulin constant like domain, cell surface, psi-biology, immune system, new york structural genomics research consortium, nysgrc, atoms-to-animals: the immune function network, ifn
• Biological source: Mus musculus (mouse)
• Total number of polymer chains: 1
• Total formula weight: 25427.01

Authors

Vigdorovich, V.,Ramagopal, U.,Bonanno, J.B.,Toro, R.,Nathenson, S.G.,Almo, S.C.,New York Structural Genomics Research Consortium (NYSGRC),Atoms-to-Animals: The Immune Function Network (IFN) (deposition date: 2012-11-16, release date: 2012-12-05, Last modification date: 2024-10-16)

Primary citation

Vigdorovich, V.,Ramagopal, U.A.,Lazar-Molnar, E.,Sylvestre, E.,Lee, J.S.,Hofmeyer, K.A.,Zang, X.,Nathenson, S.G.,Almo, S.C.
Structure and T cell inhibition properties of B7 family member, B7-H3.
Structure, 21:707-717, 2013

PubMed Abstract: T cell activity is controlled by a combination of antigen-dependent signaling through the T cell receptor and a set of auxiliary signals delivered through antigen-independent interactions, including the recognition of the B7 family of ligands. B7-H3 is a recently identified B7 family member that is strongly overexpressed in a range of cancers and correlates with poor prognosis. We report the crystal structure of murine B7-H3 at a 3 Å resolution, which provides a model for the organization of the IgV and IgC domains within the ectodomain. We demonstrate that B7-H3 inhibits T cell proliferation and show that the FG loop of the IgV domain plays a critical role in this function. B7-H3 crystallized as an unusual dimer arising from the exchange of the G strands in the IgV domains of partner molecules. This arrangement, in combination with previous reports, highlights the dynamic nature and plasticity of the immunoglobulin fold.

PubMed: 23583036
DOI: 10.1016/j.str.2013.03.003

Experimental method

X-RAY DIFFRACTION (2.97 Å)