4HYO

Crystal Structure of MthK Pore

4HYO の概要

• エントリーDOI: 10.2210/pdb4hyo/pdb
• 関連するPDBエントリー: 4HZ3
• 分子名称: Calcium-gated potassium channel mthK, POTASSIUM ION, HEXANE-1,6-DIOL, ... (4 entities in total)
• 機能のキーワード: transmembrane ion channel family, potassium channel, metal transport
• 由来する生物種: Methanothermobacter thermautotrophicus
• 細胞内の位置: Cell membrane; Multi-pass membrane protein: O27564
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 82954.56

構造登録者

Posson, D.J.,McCoy, J.G.,Nimigean, C.M. (登録日: 2012-11-13, 公開日: 2012-12-26, 最終更新日: 2023-09-20)

主引用文献

Posson, D.J.,McCoy, J.G.,Nimigean, C.M.
The voltage-dependent gate in MthK potassium channels is located at the selectivity filter.
Nat.Struct.Mol.Biol., 20:159-166, 2013

PubMed Abstract: Understanding how ion channels open and close their pores is crucial for comprehending their physiological roles. We used intracellular quaternary ammonium blockers, electrophysiology and X-ray crystallography to locate the voltage-dependent gate in MthK potassium channels from Methanobacterium thermoautotrophicum. Blockers bind in an aqueous cavity between two putative gates: an intracellular gate and the selectivity filter. Thus, these blockers directly probe gate location--an intracellular gate will prevent binding when closed, whereas a selectivity filter gate will always allow binding. Kinetic analysis of tetrabutylammonium block of single MthK channels combined with X-ray crystallographic analysis of the pore with tetrabutyl antimony unequivocally determined that the voltage-dependent gate, like the C-type inactivation gate in eukaryotic channels, is located at the selectivity filter. State-dependent binding kinetics suggest that MthK inactivation leads to conformational changes within the cavity and intracellular pore entrance.

PubMed: 23262489
DOI: 10.1038/nsmb.2473

実験手法

X-RAY DIFFRACTION (1.65 Å)