4HYJ

Crystal structure of Exiguobacterium sibiricum rhodopsin

4HYJ の概要

• エントリーDOI: 10.2210/pdb4hyj/pdb
• 分子名称: Rhodopsin, RETINAL, EICOSANE, ... (4 entities in total)
• 機能のキーワード: seven-helical transmembrane protein, proton pump, membrane, proton transport
• 由来する生物種: Exiguobacterium sibiricum
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 66308.79

構造登録者

Gushchin, I.,Chervakov, P.,Kuzmichev, P.,Popov, A.,Round, E.,Borshchevskiy, V.,Dolgikh, D.,Kirpichnikov, M.,Petrovskaya, L.,Chupin, V.,Arseniev, A.,Gordeliy, V. (登録日: 2012-11-13, 公開日: 2013-07-17, 最終更新日: 2024-10-09)

主引用文献

Gushchin, I.,Chervakov, P.,Kuzmichev, P.,Popov, A.N.,Round, E.,Borshchevskiy, V.,Ishchenko, A.,Petrovskaya, L.,Chupin, V.,Dolgikh, D.A.,Arseniev, A.A.,Kirpichnikov, M.,Gordeliy, V.
Structural insights into the proton pumping by unusual proteorhodopsin from nonmarine bacteria.
Proc.Natl.Acad.Sci.USA, 110:12631-12636, 2013

PubMed Abstract: Light-driven proton pumps are present in many organisms. Here, we present a high-resolution structure of a proteorhodopsin from a permafrost bacterium, Exiguobacterium sibiricum rhodopsin (ESR). Contrary to the proton pumps of known structure, ESR possesses three unique features. First, ESR's proton donor is a lysine side chain that is situated very close to the bulk solvent. Second, the α-helical structure in the middle of the helix F is replaced by 3(10)- and π-helix-like elements that are stabilized by the Trp-154 and Asn-224 side chains. This feature is characteristic for the proteorhodopsin family of proteins. Third, the proton release region is connected to the bulk solvent by a chain of water molecules already in the ground state. Despite these peculiarities, the positions of water molecule and amino acid side chains in the immediate Schiff base vicinity are very well conserved. These features make ESR a very unusual proton pump. The presented structure sheds light on the large family of proteorhodopsins, for which structural information was not available previously.

PubMed: 23872846
DOI: 10.1073/pnas.1221629110

実験手法

X-RAY DIFFRACTION (2.3 Å)