4HX9

Designed Phosphodeoxyribosyltransferase

4HX9 の概要

• エントリーDOI: 10.2210/pdb4hx9/pdb
• 分子名称: Nucleoside deoxyribosyltransferase, SULFATE ION, TETRAETHYLENE GLYCOL, ... (4 entities in total)
• 機能のキーワード: non natural and design enzyme, rossmann fold, phosphodeoxyribosyltransferase, transferase
• 由来する生物種: Lactobacillus leichmannii
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 146704.65

構造登録者

Kaminski, P.A.,Labesse, G. (登録日: 2012-11-09, 公開日: 2013-01-16, 最終更新日: 2023-09-20)

主引用文献

Kaminski, P.A.,Labesse, G.
Phosphodeoxyribosyltransferases, designed enzymes for deoxyribonucleotides synthesis.
J.Biol.Chem., 288:6534-6541, 2013

PubMed Abstract: A large number of nucleoside analogues and 2'-deoxynucleoside triphosphates (dNTP) have been synthesized to interfere with DNA metabolism. However, in vivo the concentration and phosphorylation of these analogues are key limiting factors. In this context, we designed enzymes to switch nucleobases attached to a deoxyribose monophosphate. Active chimeras were made from two distantly related enzymes: a nucleoside deoxyribosyltransferase from lactobacilli and a 5'-monophosphate-2'-deoxyribonucleoside hydrolase from rat. Then their unprecedented activity was further extended to deoxyribose triphosphate, and in vitro biosyntheses could be successfully performed with several base analogues. These new enzymes provide new tools to synthesize dNTP analogues and to deliver them into cells.

PubMed: 23325804
DOI: 10.1074/jbc.M112.446492

実験手法

X-RAY DIFFRACTION (2.68 Å)